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Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis 10.3791/61924-v 05:44 min
Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase 10.3791/61839-v 09:31 min
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity 10.3791/57357-v 12:31 min
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening 10.3791/53761-v 10:50 min
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor 10.3791/52504-v 07:28 min
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis 10.3791/51346-v 08:57 min
Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
13.4: Bioreactor Controls-III 01:22 min
13.4: Bioreactor Controls-III
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR 10.3791/4356-v 14:07 min
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
High-throughput Purification of Affinity-tagged Recombinant Proteins 10.3791/4110-v
High-throughput Purification of Affinity-tagged Recombinant Proteins
16.1: In vitro Mutagenesis 01:16 min
16.1: In vitro Mutagenesis
Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems 10.3791/69022-v 10:52 min
Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library 10.3791/67552-v 07:28 min
Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete 10.3791/66880-v
Procedures for In Vitro Cultivation of Treponema pallidum, the Syphilis Spirochete
Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures 10.3791/65286-v 04:01 min
Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures
Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis 10.3791/64195-v 08:19 min
Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How “Molecular Surgery” of the Backbone Affects Folding and Stability 10.3791/63320-v
Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How “Molecular Surgery” of the Backbone Affects Folding and Stability
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti 10.3791/63008-v 05:30 min
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus 10.3791/61375-v
Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis 10.3791/61365-v 10:32 min
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling 10.3791/61259-v 08:46 min
Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling
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