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JoVE Lab Manual
JoVE Journal
JoVE Encyclopedia of Experiments
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免疫学与感染
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癌症
生物学
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10.3791/67432-v
10:25 min
Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10.3791/67367-v
07:05 min
Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay
10.3791/65718-v
07:22 min
The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)
10.3791/64454-v
06:35 min
A Scalable, Cell-Based Method for the Functional Assessment of Ube3a Variants
10.3791/63602-v
10:55 min
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10.3791/63561-v
09:47 min
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
10.3791/62950-v
06:30 min
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
10.3791/62393-v
06:06 min
In Vitro Analysis of E3 Ubiquitin Ligase Function
10.3791/60702-v
08:33 min
Ubiquitin Chain Analysis by Parallel Reaction Monitoring
10.3791/60533-v
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
10.3791/60402-v
10:26 min
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
10.3791/59695-v
09:25 min
Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
10.3791/59472-v
10:44 min
Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs
10.3791/59398-v
08:40 min
Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay
10.3791/59385-v
11:36 min
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
10.3791/59079-v
11:54 min
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
10.3791/56626-v
10:40 min
Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10.3791/55949-v
12:48 min
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
10.3791/52784-v
09:45 min
Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
10.3791/52482-v
12:28 min
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
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